Genetic diversity analysis of elite pearl millet inbred lines using RAPD and SSR markers

Chandra-Shekara, A.C. and Prasanna, B.M. and Bhat, S.R. and Singh, B.B. (2007) Genetic diversity analysis of elite pearl millet inbred lines using RAPD and SSR markers. Journal of Plant Biochemistry and Biotechnology, 16 (1). pp. 23-28.

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Abstract

Pearl millet [Pennisetum glaucum (L) R Br] is one of the widely grown cereal crops in the arid and semi-arid regions of Africa nd India. We undertook a study to ascertain the genetic diversity in 21 elite inbreds (parental lines of 13 pearl millet hybrids in India) using 20 Random Amplified Polymorphic DNA (RAPD) and 21 Simple Sequence Repeat (SSR) markers. Based on Polymorphism Information Content (PIC) and unique banding profiles, 6 RAPD primers OPD12, OPA16, OPB6, OPA19, OPB5 and OPB1, and 3 SSR markers Xpsmp2208, Xpsmp2223 and Xpsmp2220, were found to be highly discriminative. The PIC values ranged from 0.28 to 0.48 for the RAPD and from 0.24 to 0.60 for the SSR markers. Cluster analysis and principal component analysis of the combined dataset of RAPD and SSR markers indicated moderate genetic divergence among the lite pearl millet germplasm, besides unraveling the genetic relationships among the male sterile lines and the restorers.

Item Type:	Article
Author Affiliation:	Division of Genetics, Indian Agricultural Research Institute, New Delhi 110 012, India
Subjects:	Crop Improvement > Plant Breeding Crop Improvement > Biotechnology
Divisions:	Millet
Depositing User:	Sushil
Date Deposited:	20 Oct 2010 09:19
Last Modified:	13 Dec 2010 20:04
Official URL:	http:\\www.IndianJournals.com
URI:	http://eprints.icrisat.ac.in/id/eprint/198

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