Evaluation of greenhouse inoculation techniques to screen sorghum for resistance to downy mildew

Narayana, Y.D. and Mughogho, L.K. and Bandyopadhyay, R. (1995) Evaluation of greenhouse inoculation techniques to screen sorghum for resistance to downy mildew. Euphytica, 86 (1). pp. 49-53.

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Abstract

Six inoculation techniques were compared for the artificial promotion of downy mildew (Peronosclerospora sorghi) in sorghum. These were (1) sprouted seeds incubated between sporulating infected leaves, (2) sprouted seeds dipped in conidial suspension, (3) sprouted seeds sprayed with conidial suspension, (4) seedlings at plumule stage inoculated with drops of a conidial suspension, (5) seedlings at plumule stage sprayed with a conidial suspension, and (6) seedling showered with conidia falling from infected leaves. Seedlings at the one-leaf stage sprayed with a conidial suspension (6 × 105 ml-1) showed the highest systemic infection (100%) in the susceptible lines IS 643 and IS 18433. This technique is effective, repeatable, and allows the deposition of a conidial suspension as a fine mist on the entire seedling surface. In the greenhouse, the technique was used to test the downy mildew reaction of genotypes previously reported as resistant (< 5% incidence) in 3-4 years of field screenings. Of the 61 genotypes tested, 21 were free from downy mildew, 14 had less than 5% incidence, and the rest showed variable susceptible reactions. Therefore, the technique can be reliably and effectively used in the greenhouse to detect disease escapes and to indentify resistance

Item Type: Article
Author Affiliation: International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh, 502 324, India
Subjects: Plant Protection > Pests
Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Sorghum
Depositing User: Mr T L Gautham
Date Deposited: 05 May 2011 03:16
Last Modified: 05 May 2011 03:16
URI: http://eprints.icrisat.ac.in/id/eprint/1959

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