Hossain, H.M. and Inden, H. and Asahira, T. (1988) Intergeneric and interspecific hybrids through in vitro ovule culture in the Cruciferae. Plant Science, 58 (1). pp. 121-128.
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Abstract
A procedure to obtain hybrid plantlets is described involving in vitro culture of ovules from hybrids of Brassica oleracea (cabbage) or B. oleracea var. alboglabra [B. alboglabra], as female parent, with B. campestris var. pekinensis [B. pekinensis], B. napus and Raphanus sativus. Modified Murashige & Skoog medium supplemented with 10% (v/v) coconut milk, 300 mg casein hydrolysate, 0.1 mg NAA and 0.1 mg kinetin (all per litre) was effective for culturing ovules. All hybrids between B. oleracea and B. campestris and between B. oleracea and R. sativus had an intermediate morphology in comparison with parental traits. Hybrids of B. oleracea and B. campestris and of B. oleracea and R. sativus were amphihaploids, as was revealed by chromosome and isoenzyme analyses
| Item Type: | Article |
|---|---|
| Author Affiliation: | Lab. Veg. & Ornamental Hort., Fac. Agric., Kyoto Univ., Kyoto 606, Japan |
| Subjects: | Plant Protection > Pests Crop Improvement > Genetics/Genomics |
| Divisions: | Other Crops |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 21 Apr 2011 09:20 |
| Last Modified: | 21 Apr 2011 09:20 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1876 |
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