Isolation and characterization of 15 microsatellite loci from mango (Mangifera indica L.) and cross-species amplification in closely related taxa

Schnell, R.J. and Olano, C.T. and Quintanilla, W.E. and Meerow, A.W. (2005) Isolation and characterization of 15 microsatellite loci from mango (Mangifera indica L.) and cross-species amplification in closely related taxa. Molecular Ecology Notes, 5 (5). pp. 625-627.

[img] PDF
Restricted to ICRISAT researchers only

Abstract

We report here on the development and characterization of 15 microsatellite loci isolated from Mangifera indica L. These markers were evaluated using 59 Florida cultivars and four related species from the USDA germplasm collection for mango. Two loci were monomorphic and 13 polymorphic, with two to seven alleles per locus. Four loci departed significantly from Hardy–Weinberg equilibrium and have significant heterozygote deficiency. Nine loci exhibited significant linkage disequilibrium. Cross-species amplification was successful in four related species. These loci are being used to investigate patterns of genetic variation within M. indica and between closely related species.

Item Type: Article
Author Affiliation: USDA-ARS-National Germplasm Repository, 13601 Old Cutler Rd., Miami, Florida 33158, USA
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 21 Apr 2011 08:10
Last Modified: 21 Apr 2011 08:10
Official URL: http://dx.doi.org/10.1111/j.1471-8286.2005.01018.x
URI: http://eprints.icrisat.ac.in/id/eprint/1871

Actions (login required)

View Item View Item