Development of microsatellite markers for mango (Mangifera indica L.)

Duval, M.F. and Bunel, J. and Sitbon, C. and Risterucci, A.M. (2005) Development of microsatellite markers for mango (Mangifera indica L.). Molecular Ecology Notes, 5 (4). pp. 824-826.

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Abstract

Microsatellite markers for mango (Mangifera indica L.) were developed using a genomic library enriched for (GA) n and (GT) n dinucleotide repeats. A subset of 41 positive clones wassequenced and primers were designed. Twenty-eight primer pairs produced polymorphic amplification products for a diversity sample including 15 mango cultivars and two accessions from the related species Mangifera laurina and Mangifera applanata. Nineteen simple sequence repeat (SSR) loci with clear scorable patterns were chosen to study diversity in the mango germplasm bank of Guadalupe (FWI). The number of alleles ranged from three to 13 with observed levels of heterozygosity ranging from 0.059 to 0.857.

Item Type: Article
Author Affiliation: UR 75 Amélioration Génétique d’Espèces à Multiplication Végétative — TA50/PS4, Avenue Agropolis, 34398 Montpellier Cedex 05, France
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 21 Apr 2011 07:35
Last Modified: 21 Apr 2011 07:35
Official URL: http://dx.doi.org/10.1111/j.1471-8286.2005.01076.x
URI: http://eprints.icrisat.ac.in/id/eprint/1870

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