Molecular cloning and functional analysis of the gene encoding geranylgeranyl diphosphate synthase from Jatropha curcas

Lin, Juan and Jin, YuanJie and Zhou, Xin and Wang, JingYa (2010) Molecular cloning and functional analysis of the gene encoding geranylgeranyl diphosphate synthase from Jatropha curcas. African Journal of Biotechnology , 9 (23). pp. 3342-3351.

[img] PDF
Restricted to ICRISAT researchers only

Abstract

Geranylgeranyl diphosphate (GGPP) synthase (GGPPS, EC: 2.5.1.29) catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic diphosphates to yield (all-E)-GGPP. GGPP is one of the key precursors in the biosynthesis of biologically significant isoprenoid compounds. Here we report for the first time the cloning of a full-length cDNA encoding GGPPS (Jc-GGPPS) from Jatropha curcas L. The full-length cDNA was 1414 base pair (bp), with an 1110-bp open reading frame (ORF) encoding a 370- amino-acids polypeptide. Bioinformatic analysis revealed that Jc-GGPPS is a member of the polyprenyltransferases with two highly conserved aspartate-rich motifs, and showed high homology to other plant GGPPSs. Phylogenetic tree analysis indicated that plant GGPPSs could be classified into two groups, angiosperm and gymnosperm, while Jc-GGPPS had closer relationship with angiosperm plant GGPPSs. Functional analysis of Jc-GGPPS in GGPPS-deficient mutant plasmid pAC-BETA (³ crtE) demonstrated that Jc-GGPPS mediated the biosynthesis of carotenoid and provided the general precursor for diterpenes biosynthesis

Item Type: Article
Author Affiliation: State Key Laboratory of Genetic Engineering, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, People’s Republic of China
Subjects: Crop Improvement
Crop Improvement > Genetics/Genomics
Divisions: Other Crops
Depositing User: Mr T L Gautham
Date Deposited: 21 Apr 2011 05:47
Last Modified: 21 Apr 2011 05:47
Official URL: http://www.academicjournals.org/AJB/PDF/pdf2010/7J...
URI: http://eprints.icrisat.ac.in/id/eprint/1866

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.