High-performance liquid chromatographic analysis of the products of linoleic acid oxidation catalyzed by pea (Pisum sativum) seed lipoxygenases

Wu, Zecai and Rabinson, D.S. and Domoney, C. and Casey, R. (1995) High-performance liquid chromatographic analysis of the products of linoleic acid oxidation catalyzed by pea (Pisum sativum) seed lipoxygenases. Journal of Agricultural and Food Chemistry, 43 (2). pp. 337-342.

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Abstract

An HPLC method is discussed for the analysis of the products formed by the pea (Pisum sativum) lipoxygenase catalyzed oxidation oflinoleic acid. The results demonstrate the feasibility of analyzing all of the hydroperoxides, hydroxides, and keto fatty acids in a single chromatographic step and show that it will be possible to analyze the product profile from the lipoxygenase activity contained in a portion of a seed, which will permit the remainder of the seed to be grown on for subsequent generations. The chemical structures of the products have been identified by HPLC analysis and GC-MS.

Item Type: Article
Author Affiliation: Department of Food Science, University of Leeds, Leeds LS2 9JT, UK
Subjects: Plant Production
Plant Protection > Pesticides
Plant Protection > Pests
Divisions: Other Crops
Depositing User: Mr B Krishnamurthy
Date Deposited: 20 Apr 2011 05:21
Last Modified: 20 Apr 2011 05:21
Official URL: http://dx.doi.org/10.1021/jf00050a014
URI: http://eprints.icrisat.ac.in/id/eprint/1806

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