Broglia, M. and Brunori, A. (1994) Synergistic effect of low temperature and high sucrose concentration on maize pollen viability in aqueous medium. Crop Science, 34 (2). pp. 528-529.
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Abstract
Maize pollen can be stored up to several hours in an inert medium such as paraffin oil. However, biological activity and genetic manipulation are almost precluded. In aqueous media, maize pollen loses viability within a few minutes when in conditions different from those promoting germination. Evidence is presented indicating that viability of maize pollen can be preserved in a hypertonic aqueous solution at 0°C. Although detrimental if individually applied, low temperature and high sucrose concentrations in combination allowed storage of maize pollen for up to 10-12 h in an aqueous medium. Such stored pollen subsequently germinated on an agar medium. The lack of in vivo fertilization by liquid pollination, at first interpreted by an osmotic hypothesis, led to the development of a new, rapid procedure for in vitro germination. Short-term storage of maize pollen in aqueous medium is now possible and should support new techniques of genetic manipulation.
| Item Type: | Article |
|---|---|
| Author Affiliation: | ENEA, Centro Ricerche Energia Casaccia, Settore Biotecnologie ed Agricoltura SP026, PO Box 2400, 00100 Rome, Italy. |
| Subjects: | Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding |
| Divisions: | Maize |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 14 Oct 2010 08:33 |
| Last Modified: | 14 Oct 2010 08:33 |
| Official URL: | https://www.crops.org/publications/cs/abstracts/34... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/18 |
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