Novel set of groundnut SSR markers for germplasm analysis and interspecific transferability

Gautami, B. and Ravi, K. and Narasu, M.L. and Hoising, D.A. and Varshney, R.K. (2009) Novel set of groundnut SSR markers for germplasm analysis and interspecific transferability. International Journal of Integrative Biology, 7 (2). pp. 100-106.

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Abstract

Cultivated groundnut (Arachis hypogaea L.), is an important oil seed crop grown mostly in tropical and subtropical regions of the world. However, it suffers with narrow genetic diversity due to genetic bottlenecks such as the recent polyploidization and self pollinating nature of the crop. In such crop species, simple sequence repeats (SSRs), as compared to other marker systems such as restriction fragment length polymorphisms (RFLPs), randomly amplified polymorphic DNAs (RAPDs) and amplified fragment length polymorphisms (AFLPs) have been found more informative for genetic studies and breeding applications. Therefore, with an aim to develop new SSR markers in groundnut, a SSR-enriched library was constructed from the genotype ICGV 86031. Out of 29 SSRs isolated, primer pairs were designed for 23 SSR loci, of which 14 (61%) primer pairs yielded scorable amplicons. Eight (57%) primer pairs showed polymorphism among 23 groundnut genotypes that are parents of different groundnut mapping populations at ICRISAT, India and EMBRAPA, Brazil. The polymorphism information content (PIC) for polymorphic SSR markers ranged from 0.13 to 0.36, with an average of 0.25. Newly isolated SSR loci showed good interspecific transferability rates ranging from 13% to 100% across seven legumes and 43% to 100% within the seven legumes. The present set of newly developed SSR markers enriches the existing groundnut SSR repertoire and the transferable SSR markers will be useful for comparative genome analysis in related legumes

Item Type: Article
Author Affiliation: International Crop Research Institute for the semi-arid tropics (ICRISAT) Hyderabad, AP, India
Subjects: Crop Improvement
Crop Improvement > Genetics/Genomics
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 14 Apr 2011 09:28
Last Modified: 14 Apr 2011 09:28
Official URL: http://ijib.classicrus.com/trns/31541302771809.pdf
URI: http://eprints.icrisat.ac.in/id/eprint/1781

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