In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gokce

Aasim, Muhammad and Day, Sibel and Rezaei, Fereshteh and Hajyzadeh, Mortaza and Mahmud, Syed Tahir and Ozcan, Sebahattin (2011) In vitro shoot regeneration from preconditioned explants of chickpea (Cicer arietinum L.) cv. Gokce. African Journal of Biotechnology , 10 (11). pp. 2020-2023.

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Abstract

The present study reports the successful shoot regeneration of preconditioned mature embryo and embryonic axis explants of chickpea cv. Gokce. Explants were preconditioned with 10 mgl benzylaminopurine (BA) for 7 days followed by culture on Murashige and Skoog (MS) medium containing 0.25, 0.50, 1.00 and 2.00 mg/l BA with or without 0.25 mg/l naphthalene acetic acid (NAA) supplemented with 4 mg/l activated charcoal and 1 mg/l polyvinylpyrrolidon (PVP). Shoot regeneration was recorded on all explants. Maximum number of (14.75) shoots per explants on mature embryo were recorded on MS medium containing 2.0 mg/l BA with 0.25 mg/l NAA. Whereas, 16.83 shoots per explants were recorded on MS medium containing 2.0 mg/l BA. Presence of NAA in the culture medium decreased the mean shoot length of both explants compared to medium devoid of 0.25 mg/l NAA. Regenerated shoots were rooted on MS medium containing 1.0 mg/l indole-butyric acid (IBA) after 4 weeks of culture. Rooted plantlets were transferred to pots for acclimatization under green house conditions.

Item Type:	Article
Author Affiliation:	Department of Biology, Kamil Ozdag Faculty of Science, Karamanoglu Mehmetbey, Yunus Emre Campus, 70200, Karaman, Turkey
Subjects:	Crop Improvement Crop Improvement > Genetics/Genomics Crop Improvement > Biotechnology
Divisions:	Chickpea
Depositing User:	Mr T L Gautham
Date Deposited:	22 Mar 2011 23:02
Last Modified:	22 Mar 2011 23:02
Official URL:	http://www.academicjournals.org/AJB/PDF/pdf2011/14...
URI:	http://eprints.icrisat.ac.in/id/eprint/1691

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