Universal primers for amplification of three non-coding regions of chloroplast DNA

Taberlet, P. and Gielly, L. and Pautou, G. and Bouvet, J. (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology, 17 (5). pp. 1105-1109.

[img] PDF
Restricted to ICRISAT researchers only

Abstract

Six primers for the amplification of three non-coding regions of chloroplast DNA via the polymerase chain reaction (PCR) have been designed. In order to find out whether these primers were universal, we used them in an attempt to amplify DNA from various plant species. The primers worked for most species tested including algae, bryophytes, pteridophytes, gymnosperms and angiosperms. The fact that they amplify chloroplast DNA non-coding regions over a wide taxonomic range means that these primers may be used to study the population biology (in supplying markers) and evolution (inter- and probably intraspecific phylogenies) of plants.

Item Type: Article
Author Affiliation: Laboratoire de Biologie et d'Evolution des Populations, Universite Joseph fourier, BP 53X, 38041, Grenoble Cedex, France
Subjects: Plant Production
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 28 Feb 2011 22:27
Last Modified: 28 Feb 2011 22:27
Official URL: http://dx.doi.org/10.1007/BF00037152
URI: http://eprints.icrisat.ac.in/id/eprint/1579

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.