Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis

Mukami, A. and Ngetich, A. and Mweu, C. and Muthangya, M. and etl, . (2018) Rapid and efficient plant regeneration from shoot apical meristems of finger millet [Eleusine coracana (L.) Gaertn.] via direct organogenesis. African Journal of Biotechnology, 17 (29). pp. 898-905.

[img]
Preview
 PDF (This is an Open Access Article) - Published Version
| Preview

Abstract

A simple and efficient plant regeneration system via direct organogenesis was established in finger millet using in vitro derived shoot apical meristems. Six varieties; GBK-043128, GBK-043094, GBK- 043050, GBK-043137, GBK-043122 and GBK-043124 were evaluated. MS medium was used for cotyledonary germination. Maximum number of shoots (84.33%) was observed in variety GBK-043128 while GBK-043094 had the least germination efficiency (62. 67%). Shoot apical meristems from threeday old seedlings were evaluated for their potency of shoot induction on varied 6-benzylaminopurine (BAP) concentrations. Highest shoot induction was observed in medium supplemented with 1.75 mg/l BAP in GBK-043050 (3.00) whereas GBK-043094 (1.28) had the least response in medium supplemented with 1.0 mg/l BAP. To induce rooting, in vitro regenerated plants cultured on MS medium was supplemented with different concentrations of indole-3- acetic acid. The highest response in root induction, with a larger number of roots (10.28), was observed in MS medium supplemented with 4.0 μM IAA. Statistical analysis indicated that plant regeneration response varied greatly among the varieties. In vitro germinated plants were successfully transferred to the greenhouse after hardening, with 300 shoots developing into fertile plants, which were indistinguishable with wild type plants. This plant regeneration system has potential for production of transgenic finger millet crops.

Item Type: Article
Uncontrolled Keywords: Direct organogenesis, finger millet, root induction, shoot apical meristems
Author Affiliation: Department of Biochemistry, South Eastern Kenya University, P. O. Box 170-90200, Kitui, Kenya
Subjects: Plant Production
Divisions: Millet
Depositing User: Mr T L Gautham
Date Deposited: 18 Jan 2019 10:59
Last Modified: 18 Jan 2019 10:59
URI: http://eprints.icrisat.ac.in/id/eprint/15780

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.