Wang, C.T. and Tang, Y.Y. and Wang, Y.Y. and Zang, X.Z. and etl, .
(2011)
Sodium azide mutagenesis resulted in a peanut plant with elevated oleate content.
Electronic Journal of Biotechnolgy, 14 (2).
pp. 1-7.
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Abstract
Screening of peanut seeds resulting from 0.39% sodium azide treatment with NIRS calibration equation for bulk seed samples identified a plant with more than 60% oleate. Oleate content in individual seeds of the plant, as predicted by NIRS calibration equation for intact single peanut seeds, ranged from 50.05% ~ 68.69%. Three seeds with >60% oleate thus identified were further confirmed by gas chromatography. Multiple sequence alignments of the FAD2B gene from Huayu 22 (wild type) and peanut seeds with elevated oleate (mutant type) revealed a C281T transition in the coding region causing an I94T substitution in the oleoyl-PC desaturase, which may be responsible for reduction in the enzyme activity.
| Item Type: |
Article
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| Uncontrolled Keywords: |
Chemical mutant, FAD2B, NIRS, oleate, peanut |
| Author Affiliation: |
Shandong Peanut Research Institute, Qingdao, China |
| Subjects: |
Crop Improvement |
| Divisions: |
Groundnut |
| Depositing User: |
Mr T L Gautham
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| Date Deposited: |
03 Jan 2019 05:37 |
| Last Modified: |
03 Jan 2019 05:37 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/15738 |
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