Chijioke, O.B. and Ifeanyi, U.M. and Blessing, A.C.
(2013)
Pollen behaviour and fertililization impairment in
Bambara groundnut (Vigna subterrenea [L.] Verdc.).
African Journal of Crop Science, 1 (3).
pp. 35-45.
![[img]](http://eprints.icrisat.ac.in/15660/1.hassmallThumbnailVersion/AfricanJCropScience_1_3_035_045_2013.pdf)  Preview |
|
PDF (This is an Open Access Article)
- Published Version
| Preview
|
Abstract
Two field experiments were conducted in April and August, 2007 cropping season at the Department of
Crop Science research farm, University of Nigeria, Nsukka, to evaluate the pollen germination
potentials and the pollen tube growth of thirteen bambara groundnut cultivars. The harvested pollen
grains were exposed for 0, 5, 10 and 15 min durations to ambient conditions before in vitro
germination. Pollen germination and pollen tube growth were tested using a medium containing 10 g
sucrose, 100 mg/l boric acid and 300 mg/l calcium nitrate made up to 100 ml with deionized water. The
results showed that cultivars, had significant effect on the pollen germination only at the late planting.
The early and late planting results showed that pollens incubated immediately after harvest had the
highest germination percentage, while pollen exposed for five minutes prior to germination showed
very poor germination. Pollens exposed beyond five minutes after harvest did not germinate. The
cultivars significantly (P < 0.05) affected the pollen tube growth at both early and late planting dates.
The pollen tube growth decreased drastically with increase in duration of pollen exposure. The pollen
tube had an exponential growth rate at the onset of the pollen tube growth, followed by lag and
stationary growth phases in the pollen tube growth curves. The exponential, logistic and Gomperzt
growth models were used to estimate the best fit model for the pollen tube growth in bambara
groundnut cultivars. In the early planting, the average straightness (R
2
) value of these models for
growth estimate were 98.4, 98.6 and 98.5%, respectively. During the late planting, the average R
2
values
were 91.5, 96.6 and 96.6%, respectively. The three models are therefore considered suitable for the
computation of the pollen tube growth rates. The principal component and cluster analyses were used
to group the cultivars in relation to the levels of pollen survival under ambient conditions. At early
planting, cultivar Bg-01 had moderate surviving pollen grains while Bg- 08, Bg-09, Bg-10 and Bg-11
were found to have poor surviving pollens. At late planting, cultivar Bg-04 and Bg-07 had high survival
pollens while Bg-01 had poor pollen survival.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
Pollen behaviour, pollination, fertilization, Bambara groundnut, Vigna subterranea (L.) Verdc |
| Author Affiliation: |
Department of Crop Science, University of Nigeria, Nsukka, Nigeria |
| Subjects: |
Crop Improvement |
| Divisions: |
Groundnut |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
29 Oct 2018 05:43 |
| Last Modified: |
29 Oct 2018 05:43 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/15660 |
Actions (login required)
 |
View Item |
