Jida, Z. and Alemu, A. and Mullualem, D.
(2018)
Genetic diversity among elite chickpea (Cicer arietinum
L.) varieties of Ethiopia based on inter simple sequence
repeat markers.
African Journal of Biotechnology, 17 (34).
pp. 1067-1075.
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Abstract
The inter simple sequence repeat (ISSR) marker was used in this study for genetic fingerprinting and
identification of released chickpea varieties and for the determination of the genetic relationships
among these cultivars. A total of 19 released chickpea varieties were subjected to ISSR analysis with
the objective of evaluating the genetic diversity among the cultivars. A total of 20 ISSR primers were
initially screened and later based on their reproducibility and polymorphism, four of them were selected
for the molecular diversity assay. Amplification of genomic DNA of the 19 varieties using four ISSR
primers produced 38 scorable fragments. On average, 9.5 polymorphic bands per primer were observed
in ISSR analysis. The total number of bands amplified by 3’ anchored primers varied from 7 to 12. The
primers based on poly (GGAGA) and (AG)YT repeat motifs produced highest number of fragments (10
and 12, respectively), whereas, primers GACA and (GA)T, produced minimum number of fragments (7
and 9, respectively). Overall, 81.58% of the loci were polymorphic and 96.17 and 3.83% of variation were
partitioned into within and among population, respectively. The least genetic similarity was recorded
between the varieties, Shasho and Minjar (0.41), and highest genetic similarity (0.97) between the
varieties, Worku and Kutaye. The UPGMA dendrogram clustered all genotypes into four different
clusters and three varieties such as Shasho, Chefe and DZ-10-11 observed to be an out lie. The results
indicate the presence of genetic diversity within released cultivars of chickpea in Ethiopia.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
Cluster analysis, gene diversity, molecular marker, polymerase chain reaction, inter simple
sequence repeat (ISSR), Ethiopia |
| Author Affiliation: |
Department of Biology, School of Natural Sciences, Wolkite University, Wolkite, Ethiopia |
| Subjects: |
Crop Improvement |
| Divisions: |
Chickpea |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
24 Sep 2018 04:46 |
| Last Modified: |
24 Sep 2018 04:46 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/15599 |
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