Elevated vitamin E content improves all-trans β-carotene accumulation and stability in biofortified sorghum

Chea, P. and Zhaoa, Z.Y. and Glassmana, K. and Doldea, D. and etl, . (2016) Elevated vitamin E content improves all-trans β-carotene accumulation and stability in biofortified sorghum. PANS, 113 (39). pp. 11040-11045.

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Abstract

Micronutrient deficiencies are common in locales where people must rely upon sorghum as their staple diet. Sorghum grain is seriously deficient in provitamin A (β-carotene) and in the bioavailability of iron and zinc. Biofortification is a process to improve crops for one or more micronutrient deficiencies.We have developed sorghum with increased β-carotene accumulation that will alleviate vitamin A deficiency among people who rely on sorghum as their dietary staple. However, subsequent β-carotene instability during storage negatively affects the full utilization of this essential micronutrient. We determined that oxidation is the main factor causing β-carotene degradation under ambient conditions. We further demonstrated that coexpression of homogentisate geranylgeranyl transferase (HGGT), stacked with carotenoid biosynthesis genes, can mitigate β-carotene oxidative degradation, resulting in increased β-carotene accumulation and stability. A kinetic study of β-carotene degradation showed that the half-life of β-carotene is extended from less than 4 wk to 10 wk on average with HGGT coexpression.

Item Type:	Article
Uncontrolled Keywords:	HGGT; biofortified sorghum; vitamin E; β-carotene accumulation; β-carotene stability
Author Affiliation:	aDuPont Pioneer, Johnston, IA 50131; and bAfrica Harvest Biotech Foundation International, Nairobi 00621, Kenya
Subjects:	Crop Improvement
Divisions:	Sorghum
Depositing User:	Mr T L Gautham
Date Deposited:	08 Aug 2018 08:07
Last Modified:	08 Aug 2018 08:07
URI:	http://eprints.icrisat.ac.in/id/eprint/15545

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