Fermentation of Sweet Sorghum syrup to butanol in the presence of natural nutrients and inhibitors

Thomas Klasson, K. and Qureshi, N. and Powell, R. and Heckemeyer, M. and etl, . (2018) Fermentation of Sweet Sorghum syrup to butanol in the presence of natural nutrients and inhibitors. Sugar Tech, 20 (3). pp. 224-234.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Request a copy

Abstract

Sweet sorghum syrups represent a renewable raw material that can be available year-round for production of biofuels and biochemicals. Sweet sorghum sugars have been used as sources for butanol production in the past but most often the studies focused on sweet sorghum juice and not on sweet sorghum syrups. Therefore, we investigated the feasibility of using the syrups as feedstock. Initial studies showed that diluted sweet sorghum syrups, to 60 g/L of glucose equivalents, could not be used as a direct replacement of a synthetic growth medium for industrial butanol-producing strain Clostridium beijerinckii. Further studies revealed that supplemental nutrients (most notably, ammonium–nitrogen) were required for successful fermentation. This was found true for two manufacturing sources of sweet sorghum syrups from commercial cultivars and hybrids. Typically, 15 g/L of total acetone, butanol, and ethanol were produced with approximately half of that being butanol. Some minor statistical difference was noted between the production potential of the two sources of syrup. However, aconitic acid, which was present at similar levels in both syrups, was ruled out as a butanol fermentation inhibitor at the fermentation pH > 4.5.

Item Type: Article
Uncontrolled Keywords: Aconitic acid,Butanol,Fermentation, Nutrients,Sweet sorghum
Author Affiliation: U.S. Department of Agriculture, Agricultural Research Service, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA
Subjects: Plant Production
Divisions: Sorghum
Depositing User: Mr T L Gautham
Date Deposited: 31 May 2018 10:49
Last Modified: 31 May 2018 10:50
URI: http://eprints.icrisat.ac.in/id/eprint/15464

Actions (login required)

View Item View Item