Diatloff, E. and Mace, E.S. and Jordan, D.R. and Filleur, S. and etl, . (2017) The vegetative nitrogen response of sorghum lines containing different alleles for nitrate reductase and glutamate synthase. Molecular Breeding, 37 (138). pp. 1-14.
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Abstract
Improving the nitrogen (N) responsiveness of crops is crucial for food security and environmental sustainability, and breeding N use efficient (NUE) crops has to exploit genetic variation for this complex trait.We used reverse genetics to examine allelic variation in two N metabolism genes. In silico analysis of the genomes of 44 genetically diverse sorghum genotypes identified a nitrate reductase and a glutamate synthase gene that were under balancing selection in improved sorghum cultivars. We hypothesised that these genes are a potential source of differences in NUE, and selected parents and progeny of nested association mapping populations with different allelic combinations for these genes. Allelic variation was sourced from African (Macia) and Indian (ICSV754) genotypes that had been incorporated into the Australian elite parent R931945-2-2. Nine genotypes were grown for 30 days in a glasshouse and supplied with continuous limiting or replete N, or replete N for 27 days followed by 3 days of N starvation. Biomass, total N and nitrate contents were quantified together with gene expressions in leaves, stems and roots. Limiting N supply universally resulted in less.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Allelic variation,Sorghum, Nested associationmapping population,Nitrogen use efficiency (NUE),Vegetative, Nitrate reductase,Glutamate synthase |
| Author Affiliation: | The University of Queensland, School of Agriculture and Food Sciences, Brisbane, QLD 4072, Australia |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 31 May 2018 10:41 |
| Last Modified: | 31 May 2018 10:41 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/15436 |
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