Xu, G.W. and Cui, Y.X. and Schertz, K.F. and Hart, G.E. (1995) Isolation of mitochondrial DNA sequences that distinguish male.sterility-inducing cytoplasms in Sorghum bicolor (L.) Moench. Theoretical and Applied Genetics, 90. pp. 1180-1187.
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Abstract
We have demonstrated that sorghum DNA sequences of mitochondrial origin can be used to distinguish different male-sterility-inducing cytoplasms. Six DNA clones containing single-copy mitochondrial sequences were hybridized on Southern blots to restriction enzymedigested DNA of 28 sorghum lines representing sources of different cytoplasmic male-sterility (CMS) groups. Four cytoplasmic types were defined on the basis of the pattern of DNA fragments detected. Similar analyses of 50 additional diverse sorghum accessions suggested that three of the four cytoplasmic types may be diagnostic for CMS. Also, three other cytoplasmic types were discovered. These and other mitochondrial DNA clones may be useful molecular tools for "fingerprinting" sterility-inducing cytoplasms in breeding programs, determining cytoplasmic diversity among germ plasm accessions, and identifying new sources of cytoplasm that induce male sterility.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Cytoplasmic male-sterility, Sorghum bicolor, Mitochondrial DNA clones |
| Author Affiliation: | Department of Soil and Crop Sciences, Texas A & M University, College Station, TX 77843 USA, and Energy Biosystems Corporation, The Woodlands, TX 77381, USA |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 08 May 2018 06:09 |
| Last Modified: | 08 May 2018 06:09 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/15431 |
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