Genome-wide identification and tissue-specific expression analysis of nucleotide binding site-leucine rich repeat gene family in Cicer arietinum (kabuli chickpea)

Sharmaa, R. and Rawatb,, V. and Suresha, C.G. (2017) Genome-wide identification and tissue-specific expression analysis of nucleotide binding site-leucine rich repeat gene family in Cicer arietinum (kabuli chickpea). Genomics Data, 14. pp. 24-31.

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Abstract

The nucleotide binding site-leucine rich repeat (NBS-LRR) proteins play an important role in the defense mechanisms against pathogens. Using bioinformatics approach, we identified and annotated 104 NBS-LRR genes in chickpea. Phylogenetic analysis points to their diversification into two families namely TIR-NBS-LRR and non- TIR-NBS-LRR. Gene architecture revealed intron gain/loss events in this resistance gene family during their independent evolution into two families. Comparative genomics analysis elucidated its evolutionary relationship with other fabaceae species. Around 50% NBS-LRRs reside in macro-syntenic blocks underlining positional conservation along with sequence conservation of NBS-LRR genes in chickpea. Transcriptome sequencing data provided evidence for their transcription and tissue-specific expression. Four cis-regulatory elements namely WBOX, DRE, CBF, and GCC boxes, that commonly occur in resistance genes, were present in the promoter regions of these genes. Further, the findings will provide a strong background to use candidate disease resistance NBS-encoding genes and identify their specific roles in chickpea

Item Type: Article
Uncontrolled Keywords: Cicer arietinum, NBS-LRR, Gene annotation Synteny DNA-motifs Gene expression
Author Affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, India
Subjects: Crop Improvement
Divisions: Chickpea
Depositing User: Mr T L Gautham
Date Deposited: 08 May 2018 06:07
Last Modified: 08 May 2018 06:07
URI: http://eprints.icrisat.ac.in/id/eprint/15427

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