Successful propagation of Pigeonpea (Cajanus cajan L.) through In vitro shoot regeneration and ex vitro rooting

Dev, A. and Kaur, A. (2017) Successful propagation of Pigeonpea (Cajanus cajan L.) through In vitro shoot regeneration and ex vitro rooting. International Journal of Biotech Trends and Technology, 23 (1). pp. 1-6.

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Abstract

A reproducible in vitro shoot regeneration protocol was developed for pigeonpea variety ICPL 87 through proliferation of axillary buds from cotyledonary nodes. The seedlings were cultured on MS + Kin (2 mgL-1) + BAP (5 mgL-1) medium and de-capitated on 7th day of culturing, which resulted in the induction of multiple shoots from cotyledonary node region after 15 days of culturing. Miniature shoots were elongated on MS + adenine suphate (30 mgL-1) medium; the elongated shoots were rooted ex vitro using a rooting powder ROOTEXTM...

Item Type: Article
Uncontrolled Keywords: Callus, Regeneration, Direct regeneration, Multiple shoots
Author Affiliation: School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, 141004, India
Subjects: Crop Improvement
Divisions: Pigeonpea
Depositing User: Mr T L Gautham
Date Deposited: 22 Mar 2018 04:05
Last Modified: 22 Mar 2018 04:05
URI: http://eprints.icrisat.ac.in/id/eprint/15389

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