Ranwez, V. and Serra, A. and Pot, D. and Chantret, N.
(2017)
Domestication reduces alternative splicing
expression variations in sorghum.
PLOS One, 12 (9).
pp. 1-20.
![[img]](http://eprints.icrisat.ac.in/15384/1.hassmallThumbnailVersion/PLOS%20ONE%20_12_9_1-20_2017.pdf)  Preview |
|
PDF (This is an open access article)
- Published Version
| Preview
|
Abstract
Domestication is known to strongly reduce genomic diversity through population bottlenecks.
The resulting loss of polymorphism has been thoroughly documented in numerous
cultivated species. Here we investigate the impact of domestication on the diversity of alternative
transcript expressions using RNAseq data obtained on cultivated and wild sorghum
accessions (ten accessions for each pool). In that aim, we focus on genes expressing two
isoforms in sorghum and estimate the ratio between expression levels of those isoforms in
each accession. Noticeably, for a given gene, one isoform can either be overexpressed or
underexpressed in some wild accessions, whereas in the cultivated accessions, the balance
between the two isoforms of the same gene appears to be much more homogenous.
Indeed, we observe in sorghum significantly more variation in isoform expression balance
among wild accessions than among domesticated accessions. The possibility exists that
the loss of nucleotide diversity due to domestication could affect regulatory elements, controlling
transcription or degradation of these isoforms. Impact on the isoform expression balance
is discussed. As far as we know, this is the first time that the impact of domestication
on transcript isoform balance has been studied at the genomic scale. This could pave the
way towards the identification of key domestication genes with finely tuned isoform expressions
in domesticated accessions while being highly variable in their wild relatives.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
Domestication, genomic diversity, population, polymorphism, sorghum |
| Author Affiliation: |
Montpellier SupAgro, UMR AGAP, Montpellier, France |
| Subjects: |
Plant Production |
| Divisions: |
Sorghum |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
22 Mar 2018 04:03 |
| Last Modified: |
22 Mar 2018 04:03 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/15384 |
Actions (login required)
 |
View Item |
