Optimization of phytohormone concentrations for callus induction in mature embryos of Finger Millet (Eleucine coracana)

Sharma, M.K. and Kashyap, A. and Singh, B.R. and Singhal, R.K. and Chauhan, J. (2017) Optimization of phytohormone concentrations for callus induction in mature embryos of Finger Millet (Eleucine coracana). International Journal Pure Applied Bioscience, 5 (4). pp. 516-525.

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Abstract

Finger millet (Eleusine coracana (L.) Gaertn.) is the primary food source for millions of people in tropical dry land regions of the world. Compared to other millet, finger millet grains also have a relatively higher content of minerals such as calcium, phosphorus, iron and manganese. It has nutritional qualities superior to that of rice and is on par with that of wheat. Finger millet is vulnerable to biotic stresses like fungal blast and abiotic stresses like drought and salinity stress. Development of efficient tissue regeneration system is an essential prerequisite for successful production of transgenic plants. For callus induction and plant regeneration in finger millet GE-3885, mature embryos were cultured onto Murashige and Skoog (MS) medium supplemented with different concentrations of 2, 4-D alone and in combination with BAP NAA and Kinetin. Callus induction was observed in all 42 different combination of phytohormone. Callus initiation was observed in 16 different combinations. Results showed that the highest callus was induced observed in MS medium containing 1.5 mg/lNAA.

Item Type: Article
Uncontrolled Keywords: 2,4-D (2,4- Di Chloro Phenoxy Acetic Acid), BAP (Benzyl Amino Purine), NAA (Nepthelic Acetic Acid), M.S Media, Callus Induction
Author Affiliation: Department of Molecular Biology and Genetic Engineering,Pantnagar, (Uttrakhand), 263145
Subjects: Crop Improvement
Divisions: Millet
Depositing User: Mr T L Gautham
Date Deposited: 22 Mar 2018 04:01
Last Modified: 22 Mar 2018 04:01
URI: http://eprints.icrisat.ac.in/id/eprint/15364

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