Garg, R. and Gupta, S. and Sengar, R.S. and Bhatnagar, S.K.
(2015)
Analysis of genetic variations in Eleusine coracana (L.) genotypes using ISSR molecular markers.
Vegetos, 28 (4).
pp. 188-193.
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Abstract
Inter Simple Sequence Repeat (ISSR)
markers were used to assess the genetic relationships
among 27 finger millet genotypes. Ten
5’ anchored and ten 3’ anchored ISSR primers
were initially screened for PCR amplifications.
From a total of 82 DNA fragments produced, 76
(92.68%) were found to be polymorphic. The
number of bands produced by different markers
was ranging from 2 to 8. PIC value and Rp value
were ranging from 0.143 to 0.983 and 0.52 to
11.56, respectively. The genetic similarity matrix
varied from 0.43 to 0.98 in these genotypes.
Dendogram analysis revealed formation of two
major groups, one with five subgroups comprising
10 genotypes and the other one consisted of
17 genotypes in five subgroups.. The results revealed
that ISSR markers could be efficiently
used to quickly access the genetic variation
available in the finger millet genotypes.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
ISSR marker, Finger millet, genetic
similarity, PIC , Rp value |
| Author Affiliation: |
Department of Molecular Biology & Genetic Engineering, College of Biotechnology, Sardar Vallabhbhai Patel
University of Agriculture & Technology, Meerut-250 110 ,U.P. |
| Subjects: |
Crop Improvement |
| Divisions: |
Millet |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
27 Dec 2017 04:07 |
| Last Modified: |
27 Dec 2017 04:07 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/15283 |
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