Shinde, V.S. and Raghuwanshi, K.S. and Chimote, V.P. and Harde, A.L. (2017) Molecular characterization of late leaf spot resistant and susceptible Groundnut (Arachis hypogaea L.) genotypes using ISSR markers. Annals of Plant Protection Sciences, 25 (1). pp. 132-139.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only Request a copy |
Abstract
Late leaf spot (LLS, Phaseoisariopsis personata) is among the major diseases causing significant yield loss in groundnut. The current study was conducted to identify diverse disease resistant genotypes for LLS for their utility in introduction into breeding programs. Among 114 groundnut genotypes (including 4 wild) screened, 12 genotypes showed resistance, 51 were moderately resistant, 41 susceptible and 10 were highly susceptible. Ten resistant genotypes were selected including two wild species viz., RHRG 6083, GPBD 4, KDG 128, ICG 11426, ICG 12672, ICG 13919, ICG 14475, ICGV 94118, ICGV 13160 (A. batizocoi) and ICGV 13165 (A. cardenasi) for molecular studies with two susceptible genotypes viz., SB XI and JL 24. Twenty two ISSRs were screened across 12 groundnut genotypes with differing levels of resistance to LLS. Overall, 12 independent clusters were formed while two wild species viz., Arachis batizicoi and A. cardenasi were found to be most divergent from other genotypes with co-efficient more than 0.51. Rest of genotypes were more similar with similarity co-efficient value more than 0.81. Two LLS susceptible genotypes SB XI and JL 24 formed separate cluster from other resistant genotypes with co-efficient more than 0.90. Four ISSR primers, ISSR 10, ISSR 14, ISSR 23 and ISSR 30 were identified marker specific to resistant genotypes. The molecular markers associated with LLS resistance can hasten the process of identification of resistant genotypes, which help groundnut breeders to design strategies for incorporating late leaf spot resistance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Arachis hypogaea, Phaseoisariopsis personata, Molecular characteristics, Resistance |
| Author Affiliation: | Seed Technology Research Unit, Seed Cell, Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722 (MS), India |
| Subjects: | Plant Protection Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 17 Oct 2017 08:40 |
| Last Modified: | 17 Oct 2017 08:40 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/15079 |
Actions (login required)
 |
View Item |
