Jency, J.P. and Ravikesavan, R. and Sumathi, P. and Raveendran, M. (2017) Effect of chemical mutagen on germination percentage and seedling parameters in Kodomillet variety Co 3. International Journal of Chemical Studies, 5 (2). pp. 166-169.
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Abstract
An experiment was conducted to estimate the lethal dose of the chemical mutagen Ethyl methyl sulphonate (EMS) in Kodomillet variety CO 3. Genetically pure seeds were treated with different doses of EMS viz., 0.2%, 0.3%, 0.4% and 0.5%. Untreated seeds were used as check for comparison. The LD50 values were observed based on growth reduction of seedlings after EMS treatment. The LD50 dose for EMS under in vitro 0.42% and in vivo condition was fixed 0.49% based on probit analysis. As the concentration of EMS increased, there was a decrease in germination, survival rate of seedlings, root length, shoot length, seedling height, vigour index under in vitro conditions and emergence and survival under field (in vivo) conditions in M1 generation as compared to the control.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Kodomillet, EMS, Lethal Dose 50 (LD 50) |
| Author Affiliation: | Agricultural University, Coimbatore - 641 003, Tamil Nadu, India |
| Subjects: | Plant Protection |
| Divisions: | Millet |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 20 Sep 2017 04:16 |
| Last Modified: | 20 Sep 2017 04:16 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/15037 |
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