Random Amplified Polymorphic DNA (RAPD) Analysis of Released Varieties and Hybrids of Pigeonpea [Cajanus cajan (L.) Millspaugh] of Gujarat

Thakar, P. and Chauhan, R.M. and Joshi, P. (2017) Random Amplified Polymorphic DNA (RAPD) Analysis of Released Varieties and Hybrids of Pigeonpea [Cajanus cajan (L.) Millspaugh] of Gujarat. International Journal of Pure and Applied Bioscience, 5 (1). pp. 674-678.

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Abstract

For diversity analysis of pigeonpea molecular marker used to study 11 diverse genotypes of pigeonpea using 12 each of RAPD primers used to determine varietal/hybrid specific informative molecular markers for precise identification of varieties and hybrid of pigeonpea. DNA was extracted as per CTAB method with minor modification and quality as analysed on nanodrop, the absorbance ratio of DNA at A260/A280 and finally with electrophoresis. Comparatively, RAPD primers generated 97 amplicons with an average 7.46 amplicons per RAPD primers. RAPD primers classified the eleven genotypes of pigeonpea into two different clusters with lot of variation in number and inclusion of genotypes in sub groups. These techniques indicated genotype GT -100 to be distantly apart from genotype GT 288 A, yet comparing other set of genotypes, GT-101 was evinced closer to GT-102 in RAPD analysis.

Item Type: Article
Uncontrolled Keywords: RAPD, DNA, Pigeonpea, Hybrids
Author Affiliation: Department of Agricultural Biotechnology, C. P. College of Agriculture
Subjects: Plant Protection
Divisions: Pigeonpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 20 Sep 2017 04:04
Last Modified: 20 Sep 2017 04:04
Official URL: http://dx.doi.org/10.18782/2320-7051.2459
URI: http://eprints.icrisat.ac.in/id/eprint/15008

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