Hess, D.E. and Ejeta, G. and Butler, L.G.
(1992)
Selecting sorghum genotypes expressing a quantitative biosynthetic trait that confers resistance to Striga.
Phytochemistry, 31 (2).
pp. 493-497.
Abstract
In laboratory experiments, all sorghums tested, regardless of whether they were susceptible or resistant to Striga (6 cultivars each), produced equivalent amounts of sorgoleone, an alkylated hydroquinone Striga germination stimulant. In contrast, some highly resistant sorghums produced relatively tiny amounts of another germination stimulant, as yet unidentified but with properties quite different from sorgoleone, whereas all susceptible sorghums produce relatively large amounts. A simple, rapid and non-destructive agar gel assay which detects this 2nd stimulant, but not sorgoleone, reliably distinguished low (Striga-resistant) and high (Striga-susceptible) stimulating parental genotypes and progenies from crosses among them. Because the lack of a reliable and rapid method for screening germplasm and breeding progenies has hampered the development of crop varieties resistant to Striga, this gel assay is expected to greatly enhance the efficiency of sorghum breeding for Striga
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