Wang, R.Y. and Wang, H.G. and Liu, X.Y. and Lian, S. and etc, . (2017) Drought-induced transcription of resistant and sensitive common millet varieties. The Journal of Animal & Plant Sciences, 27 (4). pp. 1303-1314.
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Abstract
As a well-adapted plant to dry soil and short growing seasons, common millet holds great potential as a drought-resistant crop. Here, we performed a comparative RNA-Seq analysis of two common millet genotypes, Huangmizi (tolerant) and Zhenyuandamizi (sensitive) grown under stress conditions (20% Polyethylene Glycol 6000 with an average molecular weight of ～5,400-7,000 solution). Leaves of seedling plants were harvested from a control and also after 1 and 3 h stress treatment and pooled for RNA isolation. Using Illumina paired-end sequencing, approximately 46.5 million clean reads were generated. Following a de novo assembly, a total of 42,240 Unigenes were obtained. A total of 2,301 SSRs and 1,447,148 SNPs were also identified and 75% of Unigenes were annotated. A total of 21,556 and 8,304 Unigenes aligned to the GO and COG databases, respectively. The GO classification showed that the Unigenes were distributed into three categories with 74.30% having biological functions, 76.51% having molecular functions and 79.30% having cellular-level functions. By cross-referencing against KEGGs, 5,535 Unigenes were assigned to 63 metabolic pathways. Additionally, 701 DEGs including 187 up-regulated and 514 down-regulated genes were detected. Transcripts identified in this study will accelerate our understanding of the molecular mechanisms of drought tolerance in common millet.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Common millet (Panicum miliaceum L.), Drought, Transcription |
| Author Affiliation: | College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi Province 030801 P. R. China |
| Subjects: | Crop Improvement |
| Divisions: | Millet |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 09 Aug 2017 08:36 |
| Last Modified: | 09 Aug 2017 08:36 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14923 |
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