Luo, H. and Xu, Z. and Li, Z. and et al, . (2017) Development of SSR markers and identification of major quantitative trait loci controlling shelling percentage in cultivated peanut (Arachis hypogaea L.). Theoretical and Applied Genetics, 130 (8). pp. 1635-1648.
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Abstract
Cultivated peanut (Arachis hypogaea L.) is an important grain legume providing edible oil and protein for human nutrition. Genome sequences of its diploid ancestors, Arachis duranensis and A. ipaensis, were reported, but their SSRs have not been well exploited and utilized hitherto. Shelling percentage is an important economic trait and its improvement has been one of the major objectives in peanut breeding programs. In this study, the genome sequences of A. duranensis and A. ipaensis were used to develop SSR markers, and a mapping population (Yuanza 9102 × Xuzhou 68-4) with 195 recombinant inbred lines was used to map QTLs controlling shelling percentage. The numbers of newly developed SSR markers were 84,383 and 120,056 in the A. duranensis and A. ipaensis genomes, respectively. Genotyping of the mapping population was conducted with both newly developed and previously reported markers. QTL analysis using the phenotyping data generated in Wuhan across four consecutive years and genotyping data of 830 mapped loci identified 25 QTLs with 4.46–17.01% of phenotypic variance explained in the four environments. Meta-analysis revealed five consistent QTLs that could be detected in at least two environments. Notably, the consistent QTL cqSPA09 was detected in all four environments and explained 10.47–17.01% of the phenotypic variance. The segregation in the progeny of a residual heterozygous line confirmed that the cpSPA09 locus had additive effect in increasing shelling percentage. These consistent and major QTL regions provide opportunity not only for further gene discovery, but also for the development of functional markers for breeding.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Development, SSR markers, Arachis duranensis, A. ipaensis |
| Author Affiliation: | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China |
| Subjects: | Plant Production |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 09 Aug 2017 08:29 |
| Last Modified: | 09 Aug 2017 08:29 |
| Official URL: | http://dx.doi.org/10.1007/s00122-017-2915-3 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14915 |
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