TRANSPARENT TESTA GLABRA 1 ubiquitously regulates plant growth and development from Arabidopsis to foxtail millet (Setaria italica)

Liu, K. and Qi, S. and Li, D. (2017) TRANSPARENT TESTA GLABRA 1 ubiquitously regulates plant growth and development from Arabidopsis to foxtail millet (Setaria italica). Plant Science, 254. pp. 60-69.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

TRANSPARENT TESTA GLABRA 1 of Arabidopsis thaliana (AtTTG1) is a WD40 repeat transcription factor that plays multiple roles in plant growth and development, particularly in seed metabolite production. In the present study, to determine whether SiTTG1 of the phylogenetically distant monocot foxtail millet (Setaria italica) has similar functions, we used transgenic Arabidopsis and Nicotiana systems to explore its activities. We found that SiTTG1 functions as a transcription factor. Overexpression of the SiTTG1 gene rescued many of the mutant phenotypes in Arabidopsis ttg1-13 plants. Additionally, SiTTG1 overexpression fully corrected the reduced expression of mucilage biosynthetic genes, and the induced expression of genes involved in accumulation of seed fatty acids and storage proteins in developing seeds of ttg1-13 plants. Ectopic expression of SiTTG1 restored the sensitivity of the ttg1-13 mutant to salinity and high glucose stresses during germination and seedling establishment, and restored altered expression levels of some stress-responsive genes in ttg1-13 seedlings to the wild type level under salinity and glucose stresses. Our results provide information that will be valuable for understanding the function of TTG1 from monocot to dicot species and identifying a promising target for genetic manipulation of foxtail millet to improve the amount of seed metabolites.

Item Type: Article
Uncontrolled Keywords: Arabidopsis, Foxtail millet, Seedling establishment, SiTTG1, Seed metabolitea
Author Affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China
Subjects: Crop Improvement
Divisions: Millet
Depositing User: Mr B Krishnamurthy
Date Deposited: 09 Aug 2017 08:27
Last Modified: 09 Aug 2017 08:27
Official URL: http://dx.doi.org/10.1016/j.plantsci.2016.10.010
URI: http://eprints.icrisat.ac.in/id/eprint/14906

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.