Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench

Gupta, S. and Arya, G.C. and Malviya, N. and et al, . (2016) Molecular cloning and expression profiling of multiple Dof genes of Sorghum bicolor (L) Moench. Molecular Biology Reports, 43 (8). pp. 767-774.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

DNA binding with one finger (Dof) proteins represent a family of plant specific transcription factors associated with diverse biological processes, such as seed maturation and germination, phytohormone and light mediated regulation, and plant responses to biotic and abiotic stresses. In present study, a total of 21 Dof genes from Sorghum bicolor were cloned, sequenced and in silico characterized for homology search, revealing their identity to Dof like proteins. The expression profiling of SbDof genes using quantitative RT-PCR in different tissue types and also under drought and salt stresses was attempted. The SbDof genes displayed differential expression either in their transcript abundance or in their expression patterns under normal growth condition. Two of the SbDof genes namely SbDof8 and SbDof12 showed comparatively high level of transcript abundance in all the tissue types tested; whereas some of the SbDof genes showed a distinct tissue specific expression pattern. Further a total of 13 SbDof genes showed differential expression when subjected to either of the abiotic stress i.e. drought or salinity. Three of the SbDof genes namely SbDof12, SbDof19 and SbDof24 were found to be up-regulated in response to drought and salt stress. Comparative analysis of SbDof genes expression revealed existence of a complex transcriptional and functional diversity across plant growth and developmental stages.

Item Type: Article
Uncontrolled Keywords: DNA binding with one finger (Dof), Sorghum, Expression profiling, Abiotic stress, Transcription factor
Author Affiliation: Department of Biotechnology, D.D.U Gorakhpur University, Gorakhpur, Uttar Pradesh 273 009, India
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 09 Aug 2017 08:19
Last Modified: 09 Aug 2017 08:19
Official URL: http://dx.doi.org/10.1007/s11033-016-4019-6
URI: http://eprints.icrisat.ac.in/id/eprint/14885

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.