Rapid screening assay for precise and reliable estimation of cyanide content in sorghum

Reddy, R.H. and Karthikeyan, B.J. and Agasimani, S. and et al, . (2016) Rapid screening assay for precise and reliable estimation of cyanide content in sorghum. Australian Journal of Crop Science, 10 (10). pp. 1388-1392.

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Abstract

Determining the cyanogenic potential of crop plants using traditional biochemical methods are laborious and time consuming. Presently, the Feigl-Anger paper method is being employed for qualitative analysis of cyanogenic potential of plant samples in a rapid and cost effective manner. To improve this technique from its status of qualitative to a more reliable semi-quantitative assay, the Feigl-Anger Densitometry (FAD) method was developed. This method measures the intensity of blue spots on the Feigl-Anger paper, after its exposure to HCN released from sorghum leaf tissues during a freeze thaw cycle. For colour intensity measurements, a densitometric software was employed. The densitometry results obtained from test samples and standards were compared with corresponding values obtained from quantitative spectrophotometric analysis. The results of FAD in a set of 66 germplasm lines revealed high correspondence with cyanide contents estimated through widely used spectrophotometric method of Lambert (R2=0.9107). Further, our method was also validated with known concentrations of KCN standards (R2= 0.9661). Hence it was concluded that FAD can be employed to estimate HCN content in sorghum rapidly with acceptable precision in a simple lab setup. The method can be suitable for screening of large number of mutants and segregating plant populations for the selection of low hydrogen cyanide lines in breeding programmes.

Item Type: Article
Uncontrolled Keywords: Sorghum, Cyanogenesis, Dhurrin, Feigl-Anger densitometry, Hydrogen cyanide
Author Affiliation: Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Dookie College, Dookie, VIC, 3647, Australia
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 21 Jul 2017 04:31
Last Modified: 21 Jul 2017 04:31
Official URL: http://dx.doi.org/10.21475/ajcs.2016.10.10.pne17
URI: http://eprints.icrisat.ac.in/id/eprint/14874

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