Expression of chimeric Bt gene, Cry1Aabc in transgenic pigeonpea(cv. Asha) confers resistance to gram pod borer (Helicoverpa armigera Hubner.)

Das, A. and Datta, S. and Sujayanand, G.K. and et al, . (2016) Expression of chimeric Bt gene, Cry1Aabc in transgenic pigeonpea(cv. Asha) confers resistance to gram pod borer (Helicoverpa armigera Hubner.). Plant Cell, Tissue and Organ Culture, 127 (3). pp. 705-715.

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Abstract

The gram pod borer (Helicoverpa armigera Hubner) is the most serious insect pest of pigeonpea. It is highly susceptible to the insecticidal proteins of Bacillus thuringiensis (Bt). A codon-optimized chimeric Cry1Aabc gene of Bt driven by a constitutive promoter was introduced in pigeonpea (cv. Asha) to confer resistance against the insect. A total of eight transgenic plants could be established with transformation frequency of 0.06%. Two transgenic events were selected for advancement based on high insect mortality, single locus integration, protein expression and fertility status. Quantitative ELISA indicated high protein expression in diferent plant parts viz., leaves (pre and post lowering), lowers, pod walls and immature seeds. Analysis for the stable integration, expression and insect mortality (detached leaf and pod bioassay) led to identification of lines with high efficacy. These events were further advanced for the identiication of a viable event by seling to create homozygosity. The chimeric Cry1Aabc expressed in pigeonpea is efective against gram pod borer and can be utilized in transgenic variety development programme.

Item Type: Article
Uncontrolled Keywords: Transgenic pigeonpea, Cajanus cajan L., Genetic transformation, Agrobacterium, Helicoverpa armigera, Cry1Aabc gene, Bacillus thuringiensis
Author Affiliation: Division of Plant Biotechnology, ICAR-Indian Institute of Pulses Research, Kanpur 208 024, India
Subjects: Plant Protection
Divisions: Pigeonpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 21 Jul 2017 03:41
Last Modified: 21 Jul 2017 03:41
Official URL: http://dx.doi.org/10.1007/s11240-016-1131-1
URI: http://eprints.icrisat.ac.in/id/eprint/14839

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