High throughput transformation of a Sorghum cDNA library for rice improvement

Qin, K. and Qiu, P. and Wen, J. and et al, . (2016) High throughput transformation of a Sorghum cDNA library for rice improvement. Plant Cell, Tissue and Organ Culture, 125 (3). pp. 471-478.

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Abstract

The improvement of rice productivity is an important subject due to great population pressure. As plant functional genomics rapidly develops, increasing numbers of genes from different organisms are documented for rice improvement. Sorghum, a relative of rice, has many novel phenotypic characteristics such as strong stature, large panicle, drought tolerance, and a tolerance for low soil fertility, making this species an excellent gene resource for rice improvement. A Sorghum cDNA library was transformed into rice generating an overexpression population with 1153 fertile transgenic lines. Over 900 of these lines displayed phenotypic changes including: plant height, leaf shape and size, effective panicles, and other vegetative characteristics. Importantly, there were 19 lines with higher grain yield than the wild type, which demonstrates that the high-throughput transformation of overexpressing transgenes from Sorghum into rice is a highly efficient method for rice improvement.

Item Type: Article
Uncontrolled Keywords: cDNA library, Sorghum, Rice, Genetic improvement, Alien expression
Author Affiliation: State Key Laboratory of Hybrid Rice, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Sciences, Wuhan University, Wuhan 430072, China
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 21 Jul 2017 03:26
Last Modified: 21 Jul 2017 03:26
Official URL: http://dx.doi.org/10.1007/s11240-016-0962-0
URI: http://eprints.icrisat.ac.in/id/eprint/14824

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