Direct organogenesis from cotyledonary node explants suitable for Agrobacterium-mediated transformation in peanut (Arachis hypogaea L.)

Hsieh, Y. and Jain, F.M. and Wang, J. and Gallo, M. (2017) Direct organogenesis from cotyledonary node explants suitable for Agrobacterium-mediated transformation in peanut (Arachis hypogaea L.). Plant Cell, Tissue and Organ Culture, 128 (1). pp. 161-175.

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Abstract

Efficient and genotype-independent in vitro regeneration is an essential prerequisite for incremental trait improvement in peanut (Arachis hypogaea L.) via genetic transformation. We have optimized a facile and rapid method to obtain direct shoot organogenesis from cotyledonary node (CN) explants excised from peanut seedlings germinated on cytokinin-supplemented Murashige and Skoog (MS) basal salt medium. Starting with mature embryos, shoot induction occurred in approximately 7 weeks, followed by 4 weeks for rooting of excised shoots and 3 weeks of acclimatization of regenerated plantlets in soil.......

Item Type: Article
Uncontrolled Keywords: Agrobacterium-mediated transformation, Arachis hypogaea, Cotyledonary nodes, Cytokinins, Direct organogenesis
Author Affiliation: Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32610-3610, USA
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr B Krishnamurthy
Date Deposited: 01 Mar 2017 06:11
Last Modified: 01 Mar 2017 06:11
Official URL: http://dx.doi.org/10.1007/s11240-016-1095-1
URI: http://eprints.icrisat.ac.in/id/eprint/14773

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