Umar, I.D. and Kwon-Ndung, E.H. and Mailafia, S.
(2016)
Genotypic restriction patterns of finger millet (Eleusine
coracana (l.) Gaertn) landraces in northern Nigeria.
International Journal of Genetics and Molecular Biology, 8 (2).
pp. 7-10.
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Abstract
The present study was conducted to characterize the genetic diversity of 10 germplasm accessions of
finger millet (Eleusine coracana (L) Gaertn) collected from diverse locations spread across the
geographical zone of Northern Nigeria. The digest was carried out using the molecular marker
Restriction Fragment Digest (RFD on agarose gel while EcoR1 and Hind III restriction enzymes were
used to cut the genomic DNA at specific sites. The results of our findings using RFD generated four
clear DNA bands of molecular weights ranging from 10000, 8500, 1000 and 200 KDA. This clearly
suggests the existence of polymorphism among the plant accessions. These results demonstrate the
high variability that exists amongst the genetic traits for these germplasm accessions. Information on
this plant is very useful in unraveling the pedigree genetic relationships, as well as, in designing
breeding and selection experiments for improvement of this crop. It will further stimulate growing
interest on the genetic diversity and classification of this crop.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
Genotypic, restriction digestion, finger millet, Eco R1, Hind 111, northern Nigeria |
| Author Affiliation: |
Department of Biological Sciences, University of Abuja, Abuja Nigeria |
| Subjects: |
Crop Improvement |
| Divisions: |
Millet |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
10 Jan 2017 06:49 |
| Last Modified: |
10 Jan 2017 06:49 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/14660 |
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