In vitro regeneration of chickpea (Cicer arietinum L.) via somatic embryogenesis

Shukla, A. and Das, A. and Ansari, A. and et al, . (2015) In vitro regeneration of chickpea (Cicer arietinum L.) via somatic embryogenesis. Journal of Food Legumes, 28 (3). pp. 199-202.

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Abstract

Somatic embryogenesis was induced from calli derived from embryonic axis explants of chickpea (Cicer arietinum L.). Five different concentrations of the auxin, 2,4- dichlorophenoxyaceticacid (2,4-D) was tested for induction of embryogenetic potential from explants. Explants cultured in modified MS (Murashige and Skoog) medium containing 3.0 mgl-1 2,4-D responded best in terms of callus induction (82%), conversion of calli to embryogenetic pathway and finally plantlet development. Developed plantlets exhibited healthy shoots and roots that could be successfully hardened in soil and established as mature fertile plants. The reported protocol can be effectively utilized for genetic improvement of chickpea.

Item Type: Article
Uncontrolled Keywords: Chickpea, Somatic embryo, Embryonic axes, Regeneration
Author Affiliation: Biotechnology Unit, Indian Institute of Pulses Research, Kanpur 208 024
Subjects: Plant Protection
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 17 Dec 2016 03:46
Last Modified: 17 Dec 2016 03:46
URI: http://eprints.icrisat.ac.in/id/eprint/14647

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