Kumar, A. and Sharma, D. and Tiwari, A. and Jaiswal, J.P. and Singh, N.K. and Sood, S.
(2016)
Genotyping-by-sequencing analysis for determining population structure of Finger Millet germplasm of diverse origins.
The Plant Genome, 9 (2).
pp. 1-15.
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Abstract
Finger millet [Eleusine coracana (L.) Gaertn.] is grown mainly by
subsistence farmers in arid and semiarid regions of the world.
To broaden its genetic base and to boost its production, it is of
paramount importance to characterize and genotype the diverse
gene pool of this important food and nutritional security crop.
However, as a result of nonavailability of the genome sequence
of finger millet, the progress could not be made in realizing
the molecular basis of unique qualities of the crop. In the present
investigation, attempts have been made to characterize the
genetically diverse collection of 113 finger millet accessions
through whole-genome genotyping-by-sequencing (GBS), which
resulted in a genome-wide set of 23,000 single-nucleotide
polymorphisms (SNPs) segregating across the entire collection
and several thousand SNPs segregating within every accession.
A model-based population structure analysis reveals the presence
of three subpopulations among the finger millet accessions,
which are in parallel with the results of phylogenetic analysis. The
observed population structure is consistent with the hypothesis
that finger millet was domesticated first in Africa, and from there
it was introduced to India some 3000 yr ago. A total of 1128
gene ontology (GO) terms were assigned to SNP-carrying genes
for three main categories: biological process, cellular component,
and molecular function. Facilitated access to high-throughput genotyping
and sequencing technologies are likely to improve the
breeding process in developing countries, and as such, this data
will be very useful to breeders who are working for the genetic
improvement of finger millet.
| Item Type: |
Article
|
| Uncontrolled Keywords: |
Finger Millet, Germplasm, Population, Genetic,genotyping |
| Author Affiliation: |
Dep. of Molecular Biology &
Genetic Engineering, G.B. Pant Univ. of Agriculture and Technology, Pantnagar, Uttarakhand 263145 |
| Subjects: |
Crop Improvement |
| Divisions: |
Millet |
| Depositing User: |
Mr T L Gautham
|
| Date Deposited: |
06 Dec 2016 07:34 |
| Last Modified: |
06 Dec 2016 07:34 |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/14609 |
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