Sahu, A.R. and Mishra, R.R. and Panigrahi, J. (2016) Estimation of genetic diversity among 34 genotypes in the genus Cajanus with contrasting host response to the pod borer and its allied pests. Journal of Crop Science and Biotechnology, 19 (1). pp. 17-35.
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Abstract
The genetic divergence among 34 genotypes belonging to 12 species of genus Cajanus were carried out using plant pest interaction and DNA marker analysis. Principal component analysis based on average percentage of pod damage caused by pod borer, plume moth, and blue butterfly in the field conditions, and growth of their larva and pupa on an artificial diet in vitro dispersed these genotypes into four coordinates evincing high genetic divergence as expected. DNA marker analysis using 11 pairs of SSR and nine ISSR primers showed higher polymorphism at the species level, and these primers exhibited variation with regard to average band informativeness, resolving power, and PIC value. No single primer was able to distinguish between all the 34 genotypes of Cajanus but nine species specific amplified fragments were generated by five ISSR primers. The pairwise Jaccard’s similarity coefficient and Nei’s genetic distance values revealed a higher level of inter-specific genetic variation in the genus Cajanus. The clustering of genotypes based on Jaccard’s similarity coefficient vis-a-vis Nei’s genetic distance agreed with the sectional classification of the genus Cajanus. Seven cultivars of C. cajan and the genotypes of their wild progenitor C. cajanifolius remained in one cluster, whereas accessions of C. platycarpus and C. scarabaeoides were out grouped. The rest of the genotypes belonging to nine species of Cajanus formed another cluster. The principal coordinate analysis also supported this clustering pattern. Moreover, these findings have good many implications for future breeding endeavors aimed at the introgression of pod borer resistance alleles.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Dendrogram, PCA, pigeonpea, plant pest interaction, pod borer |
| Author Affiliation: | Plant Biotechnology Laboratory, School of Life Sciences, Sambalpur University, Jyoti Vihar, Burla-768019, Sambalpur, Odisha, India |
| Subjects: | Crop Improvement |
| Divisions: | Pigeonpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 22 Nov 2016 06:11 |
| Last Modified: | 22 Nov 2016 06:11 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14592 |
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