Molecular analysis of the chloroplast Cu/Zn-SOD gene (AhCSD2) in peanut

Zhang, X. and Wan, Q. and Liu, F. and Zhang, K. and etl, . (2015) Molecular analysis of the chloroplast Cu/Zn-SOD gene (AhCSD2) in peanut. Crop Journal, 3. pp. 246-257.

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Abstract

Superoxide dismutase (SOD, EC 1.15.1.1) plays a key role in response to drought stress, and differences in SOD activity changes among cultivars are important under drought conditions. We obtained the full-length DNA of the chloroplast Cu/Zn-SOD gene (AhCSD2) from 11 allotetraploid cultivars and 5 diploid wild species in peanut. BLAST search against the peanut genome showed that the AhCSD2 genes gCSD2-1 and gCSD2-2 are located at the tops of chromosome A03 (A genome) and B03 (B genome), respectively, and both contain 8 exons and 7 introns. Nucleotide sequence analyses indicated that gCSD2-2 sequences were identical among all the tested cultivars, while gCSD2-1 sequences showed allelic variations. The amino acid sequences deduced from gCSD2-1 and gCSD2-2 both contain a chloroplast transit peptide and are distinguished by 6 amino acid (aa) residue differences. The other 2 aa residue variations in the mature peptide regions give rise to three-dimensional structure changes of the protein deduced from the genes gCSD2-1 and gCSD2-2. Sequences analyses of cultivars and wild species showed that gCSD2-2 of Arachis hypogaea and gAipCSD2 (Arachis ipaensis) are identical, and despite the abundant polymorphic loci between gCSD2-1 of A. hypogaea and sequences from A genome wild species, the deduced amino acid sequence of AhCSD2-1 (A. hypogaea) is identical to that of AduCSD2 (Arachis duranensis), whereas AcoCSD2 (Arachis correntina) and AcaCSD2 (Arachis cardenasii) both have 2 aa differences in the transit peptide region compared with AhCSD2-1 (A. hypogaea). Based on the Peanut Genome Project, promoter prediction revealed many stress-related cis-acting elements within the potential promoter regions (pp-A and pp-B). pp-A contains more binding sites for drought-associated transcriptional factors than pp-B. We hypothesize that the marked changes in SOD activity in different cultivars under drought stress are tightly regulated by transcription factors through transcription and expression of AhCSD2 genes.

Item Type: Article
Uncontrolled Keywords: Peanut, SOD activity, AhCSD2 gene, Bioinformatics analysis, Drought resistance
Author Affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an 271018, China
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 22 Nov 2016 06:06
Last Modified: 22 Nov 2016 06:06
URI: http://eprints.icrisat.ac.in/id/eprint/14578

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