Rapid and efficient Agrobacterium-mediated transformation of sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker

Do, D.P. and Lee, H. and Mookkan, M. and et al, . (2016) Rapid and efficient Agrobacterium-mediated transformation of sorghum (Sorghum bicolor) employing standard binary vectors and bar gene as a selectable marker. Plant Cell Reports, 35 (10). pp. 2065-2076.

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Abstract

A rapid and efficient Agrobacterium -mediated transformation system in sorghum has been developed employing standard binary vectors and bar gene as a selectable marker. Sorghum (Sorghum bicolor) is an important food and biofuel crop worldwide, for which improvements in genetic transformation are needed to study its biology and facilitate agronomic and commercial improvement. Here, we report optimization of regeneration and transformation of public sorghum genotype P898012 using standard binary vectors and bar gene as a selectable marker. The tissue culture regeneration time frame has been reduced to 7-12 weeks with a yield of over 18 plants per callus, and the optimized transformation system employing Agrobacterium tumefaciens strain AGL1 and the bar with a MAS promoter achieved an average frequency over 14 %. Of randomly analyzed independent transgenic events, 40-50 % carry single copy of integrated T-DNA. Some independent transgenic events were derived from the same embryogenic callus lines, but a 3:1 Mendelian segregation ratio was found in all transgenic events with single copy as estimated by Southern blots. The system described here should facilitate studies of sorghum biology and agronomic improvement.

Item Type: Article
Uncontrolled Keywords: Agrobacterium, Sorghum, Plant transformation, Standard binary vectors, Bar
Author Affiliation: Plant Transformation Core Facility, Division of Plant Sciences, University of Missouri, 1-33 Agriculture Building, Columbia, MO 65211, USA
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 14 Nov 2016 10:45
Last Modified: 14 Nov 2016 10:45
Official URL: http://dx.doi.org/10.1007/s00299-016-2019-6
URI: http://eprints.icrisat.ac.in/id/eprint/14543

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