Jayasena, S. and Smits, M. and Fiechter, D. and etl, . (2015) Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major Peanut allergens. Journal of Agricultural and Food Chemistry, 63. pp. 1849-1855.
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Abstract
Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Peanut, allergens, Ara h 1, Ara h 2, Ara h 3, Ara h 6, immunoassay, ELISA, detection |
| Author Affiliation: | Food Allergy Research and Resource Program, Department of Food Science & Technology, University of Nebraska, 143 Food Industry Complex, Lincoln, Nebraska 68583-0919, United States |
| Subjects: | Plant Protection |
| Divisions: | Groundnut |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 28 Oct 2016 06:43 |
| Last Modified: | 28 Oct 2016 06:43 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14479 |
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