Several cis-elements including a palindrome involved in pollen-specific activity of SBgLR promoter

Zhou, P. and Yang, F. and Yu, J. and Ao, G. and Zhao, Q. (2010) Several cis-elements including a palindrome involved in pollen-specific activity of SBgLR promoter. Plant Cell Reports, 29 (5). pp. 503-511.

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Abstract

Abstract SBgLR (Solanum tuberosum genomic lysinerich) is a pollen-specific gene cloned from potato (Solanum tuberosum L.). The region from -269 to -9 (The A of translation start site ‘‘ATG’’ as ?1) of the SBgLR promoter was identified as critical for gene specific expression in pollen grains. Sequence analysis indicates a palindromic sequence ‘‘TTTCTATTATAATAGAAA’’ in the -227 to -209 region, in which two pollen-specific motifs TTTCT and AGAAA surround a unique putative TATA box. Moreover, nine putative pollen-specific motifs are located in the region between the TATA box and ATG. We placed the -227 to -9 region (reserving the palindrome) and the -222 to -9 region (breaking the palindrome) downstream of the CaMV35S enhancer, respectively, to construct two fusion promoters. Histochemical assays in transgenic plants demonstrated that the region from -222 to -9 is necessary and sufficient for pollen-specific expression of the uidA gene. However, the region of -227 to -9 is incapable of driving GUS expression in pollen grains and parts of vegetative tissues. A series of 50 deletions from -269 to -9 of SBgLR promoter were constructed. A transient expression assay indicated that the region from the -227 to -9 suppressed gfp gene expression in pollen, and a positive regulatory element was present in the region of -253 to -227. The function of the palindromic sequence as a repressor inhibiting gene expression in pollen was further confirmed by the mutated promoter, breaking the palindrome by substituting its 30-flanking five base pairs, which resumes the reporter gene expression in mature pollen

Item Type:	Article
Subjects:	Crop Improvement
Divisions:	Other Crops
Depositing User:	Syamala
Date Deposited:	02 Feb 2011 22:14
Last Modified:	02 Feb 2011 22:14
Official URL:	http://dx.doi.org/10.1007/s00299-010-0839-3
URI:	http://eprints.icrisat.ac.in/id/eprint/1445

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