Mohamed, G.I.A. and Mohamed, G.A.A. and Mohamed, M.S. and Abo-Elnaga, H.I.G. and Mahran, A. (2016) Genetic diversity among isolates of Fusarium spp causing root rot and damping off Chick-Pea. International Journal of Agriculture Innovations and Research, 4 (5). pp. 857-864.
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Abstract
Species of Fusarium causes root rot and damping-off diseases in chickpea in Egypt. Twenty three Fusarium isolates were collected from diseased chick pea plants with root rot and damping off symptoms from different chickpea fields located at Assiut governorate in Egypt and they were tested for their pathogenicity. All tested isolates were infected chickpea plants of Giza 3 cultivar and caused root rot and damping off. The tested fungal isolates were varied in their virulence. Fusarium oxysporum isolate Nr 13 caused the highest percentage of post emergence damping off and root rot (40% and 100%, respectively) followed by F. oxysporum Nr 6 (30% and 92.5%, respectively). The lowest percentage of pre and post emergence damping off and root rot showed with Fusarium oxysporum isolate Nr 17 (30%, 2% and 31.3%, respectively) followed by F. solani Nr 19 (28%, 6% and 32.5%, respectively). The isolates were paired in all possible combinations for determined vegetative compatibility and non-compatibility between the isolates of Fusarium sp. where some isolates shown compatible and incompatible reactions with all other isolates on CZ medium at 28 oC. While, some isolates shown an exciting result, where it shown self incompatibility reaction. Only nine isolates, belong to Fusarium oxysporum, Fusarium solani and Fusarium moniliform were subjected to molecular investigation. Polymorphism and genetic similarity among the nine Fusarium fungi isolates revealed by RAPD markers were obtained using three arbitrary oligonucleotide primers. The number of the amplified fragments per primer varied between one and seventeen giving a total of 192 bands in all samples, with an average 64 bands per primer. These fragments have a size ranged from 4206 to 94 base pairs. Only two bands from a total number of 192 amplified bands were being monomorphic and the other 190 bands were polymorphic with a polymorphism ratio of 98.96 %. The primers used generated unique distinct band which could be used to distinguish the isolates in respect of their species and virulence. Fragments with molecular weight of 2698 bp and 1060 bp could be used as genetic markers (absent and present, respectively) to distinguish F. oxysporum Nr 13, which considered the sever pathogenic isolate. Also fragments of 1683, 1336 and 389 bp were present only as a positive marker in F. solani Nr 18, which considered the sever pathogenic isolate. The genetic relationships between the nine isolates of Fusarium was divided all samples into three main clusters as shown in phylogenetic tree. The results confirming that the evolution direction draws line from F. moniliform toward Fusarium oxysporum. As well as, the results asserted that RAPD-PCR technique is useful tool for differentiating between species of the genus Fusarium.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Fusarium sp, Fusarium Pathogenicity, Vegetative Compatibility, Fusarium Fingerprinting and RAPD-PCR of Fusarium |
| Author Affiliation: | Genetic department, Faculty of Agriculture, Assiut University, Assiut-Egypt |
| Subjects: | Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 23 Aug 2016 07:57 |
| Last Modified: | 23 Aug 2016 07:57 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14328 |
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