Farahani, S. and Morid, B. and Maleki, M and Saberi, S. (2015) Using SCAR molecular marker to detect resistance genes to Fusarium oxysporum f. sp. ciceris in chickpea cultivars and lines. Biological Forum – An International Journal, 7 (1). pp. 1369-1376.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only Request a copy |
Abstract
The occurrence of fusarium wilt disease is reported from 32 countries around the world. It is estimated that this disease results in 10-90% crop yield loss annually. It is difficult to control this disease because of the ability of the pathogen to survive in soil for several years, even without the host. The most effective method for controlling fusarium wilt is the cultivation of resistant chickpea cultivars. In this experiment, SCAR molecular marker was used to detect chickpea genotypes resistant to the disease. The DNA of 42 chickpea genotypes was extracted by CTAB method. After that, polymerase chain reaction was conducted by CS-27 and OPM-20 molecular markers. Results showed that 41 genotypes out of the 42 tested genotypes were sensitive to all five races of the pathogen. The only resistant genotype was Flip 06-152c which was resistant to the five races. Finally, the pathogenesis test confirms all results obtained in the genetic studies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chickpea fusarium wilt disease, SCAR |
| Author Affiliation: | Department of Plant Protection, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran |
| Subjects: | Crop Improvement |
| Divisions: | Chickpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 23 Aug 2016 07:56 |
| Last Modified: | 23 Aug 2016 07:56 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14325 |
Actions (login required)
 |
View Item |
