Molecular diversity assessment in chickpea through RAPD and ISSR markers

Bhagyawant, S.S. and Gupta, N. and Gautam, A. and Chaturvedi, S.K. and Shrivastava, N. (2015) Molecular diversity assessment in chickpea through RAPD and ISSR markers. World Journal of Agricultural Research, 3 (6). pp. 192-197.

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Abstract

Chickpea (Cicer arietinum L.) is a cool season food legume in the family of Fabaceae. The exploration of genetically variable accessions is the key source of germplasm conservation for future selecting parents for breeding. PCR based analysis employing RAPD and ISSR marker are one of the established techniques of estimation of genetic diversity. Twelve chickpea accessions were analyzed by ten RAPD and ISSR primers of which all primers gave amplification products. The results showed that the level of DNA polymorphism in these accessions is low. RAPD analysis yielded 5.9 bands per primer while ISSR analysis produce 2.8 bands per primer. The average percentage of molecular polymorphism as produced by RAPD and ISSR primers was 67%. The cluster dendrogram demonstrated a similarity coefficient range of 0.10 to 0.66 due to RAPD markers, whereas ISSR markers showed 0.60 to 0.80. The variability index worked out in the present study ranges from 0.49 to 0.78. The present study determines DNA polymorphism in chickpea accessions as revealed by RAPD and ISSR markers. Overall the study ascertained that RAPD and ISSR marker provide powerful tools in revealing genetic diversity in chickpea.

Item Type:	Article
Uncontrolled Keywords:	Chickpea, RAPD, ISSR, polymorphism
Author Affiliation:	School of Studies in Biotechnology, Jiwaji University, Gwalior, India
Subjects:	Crop Improvement
Divisions:	Chickpea
Depositing User:	Mr T L Gautham
Date Deposited:	23 Aug 2016 07:56
Last Modified:	23 Aug 2016 07:56
URI:	http://eprints.icrisat.ac.in/id/eprint/14324

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