Babu, B.K. and Agrawal, P.K. and Pandey, D. and Sood, S. and etl, . (2014) Molecular analysis of world collection of finger millet accessions for blast disease resistance using functional SSR markers. SABRAO Journal of Breeding and Genetics, 46 (2). pp. 202-216.
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Abstract
The major limiting factor for production and productivity of finger millet is blast disease caused by Magnaporthe grisea. In the present study, most of the exotic genotypes showed highly resistant to moderately resistant reaction to neck blast and finger blast disease. Neck blast found to have significant and positive correlation with finger blast (0.612**), but a poor correlation was observed with leaf blast (0.08**). Fifty-eight genic SSRs were used for genetic diversity analysis of a global collection of 190 finger millet genotypes. Three SSR markers (RM23842, RM5963 and RM262) were found to be most highly polymorphic loci for differentiating the 190 global collections of finger millet genotypes. The SSR marker RM5963 containing the trimer repeat motif CAG showed highest PIC values, followed by tetra- (RM23842) and di- (RM262) repeat motif containing SSRs. The 58 genic SSR loci of blast disease grouped the 190 finger millet genotypes into 4 major clusters based on their blast disease response by phylogenetic clustering as revealed by Power Marker software. The average gene diversity existing among all the inbred lines was relatively high (49%), indicating existence of high levels of polymorphisms among the finger millet. Among the genotypes of NW Himalayan region of India, VHC3997 and VHC3930 found highly resistant to neck blast which can be used as donor parents for production of blast resistant finger millet varieties.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Finger millet, blast disease, comparative genomics, EST-SSR markers, diversity, power marker |
| Author Affiliation: | Department of Molecular Biology and Genetic Engineering, College of Basic Sciences & Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, India |
| Subjects: | Crop Improvement |
| Divisions: | Millet |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 10 Mar 2016 05:31 |
| Last Modified: | 10 Mar 2016 05:31 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/14225 |
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