Khan, R. and Khan, H. and Farhatullah, . and Harada, K. (2010) Evaluation of microsatellite markers to discriminate induced mutation lines, hybrid lines and cultigens in chickpea (Cicer arietinum L). Autralian Journal of Crop Science, 4 (5). pp. 301-308.
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Abstract
A total of 47 chickpea (Cicer arietinum L) accessions including 21 induced mutation lines, 17 hybrid lines, 5 local cultigens, and 4 non-nodulating lines were examined for their growth characteristics in the field. Genotypes of these accessions were also examined using 10 SSR (microsatellite) markers. Eight of the 10 markers were polymorphic and the number of alleles ranged from 2 to 16, with the average of 7.4 per locus. PIC values ranged from 0.227 to 0.876, with an average of 0.636. The accessions comprised 29 Desi and 18 Kabuli phenotypes. The average PIC was 0.582 in Desi and 0.577 in Kabuli phenotypes. Significant genetic differentiation was revealed between Desi and Kabuli phenotypes by AMOVA under stepwise mutation assumption (RST = 0.239, P ≤ 0.001). A considerable amount of genetic variation was observed in the mutation accumulation lines tested. PIC for the accessions derived from the Desi parental line C-44 was 0.443 and that of Pb-91 was 0.422, which accounts for about 66 to 70% of the total variation within our collection, respectively. UPGMA and ME trees classified the accessions into 6 groups and all but 6 accessions could be clearly separated. Grouping was mostly the same in the two phylogenetic trees, but the branching order differed greatly. Recent introgression among the parental lines is suggested for this reason. Our study indicates that SSR markers are useful tools for discriminating induced mutation lines as well as hybrid lines.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Plant Breeding and Genetics, NWFP Agriculture University, Peshawar, Pakistan |
| Subjects: | Crop Improvement > Plant Breeding Crop Improvement > Biotechnology |
| Divisions: | Chickpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 20 Oct 2010 09:28 |
| Last Modified: | 29 Dec 2010 19:56 |
| Official URL: | http://www.cropj.com/harada_4_5_2010_301_308.pdf |
| URI: | http://eprints.icrisat.ac.in/id/eprint/142 |
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