Genetic transformation in the grain legume Cicer arietinum L. (chickpea)

Fontana, G.S. and Santini, L. and Caretto, S. and Frugis, G. and Mariotti, D. (1993) Genetic transformation in the grain legume Cicer arietinum L. (chickpea). Plant Cell Reports, 12 (4). pp. 194-198.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Request a copy

Abstract

In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for A~robacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase II and Bglucuronidase activities and the presence in their genome of integrated bacterial DNA.

Item Type: Article
Uncontrolled Keywords: Biotechnology, Genetic transformation,Chickpeas,Grain legumes,Tissue culture
Author Affiliation: Istituto di Radiobiochimica ed Ecofisiologia Vegetali, CNR, via Salaria Km 29.300, 00016 Monterotondo scalo (RM), Italy.
Subjects: Plant Production
Divisions: Chickpea
Depositing User: Mr T L Gautham
Date Deposited: 24 Feb 2016 08:06
Last Modified: 24 Feb 2016 08:06
URI: http://eprints.icrisat.ac.in/id/eprint/14199

Actions (login required)

View Item View Item